Michael Martyn

Letters B, J, O, U, X and Z have no amino acid, so they show in grey

e.g. michael = ATG·ATA·TGC·CAT·GCA·GAA·CTT

All living things have DNA. DNA is like an instruction manual that tells our bodies how to grow and work properly. Sometimes scientists want to find out what a piece of DNA does or how it works. But they may not know what the end result should look like.

A biological process called 'reverse translation' helps here. It is like looking at a finished cake and working out the ingredients that made it. Usually you start with the ingredients and make the cake. Here you start with the cake and work out the ingredients. In reverse translation, scientists start with a protein that they want to understand. Then they try to find the DNA sequence that makes it. It is like doing the steps of making a protein in reverse order

Written by Michael Martyn. Edited by Claude. "Explain reverse translation like I'm 10"
What

DNA uses an alphabet of 4 letters (A, T, C, G). Proteins use an alphabet of 20 letters (A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y). Each protein letter is one amino acid. DNA bases group into sets of 3, called "codons". A codon is the link between DNA and protein. For example, the letter "M" is the amino acid Methionine. The codon ATG codes for it, and ATG contains the 3 DNA bases A, T and G

How

This app uses JavaScript to convert the letters that you type into a DNA sequence. It treats each letter as an amino acid and looks up a codon for it. In reverse mode, it splits a DNA string into codons of 3 bases. Then it looks up the amino acid for each codon

A restriction enzyme cuts DNA at one short sequence, called a restriction site. Most sites are palindromes, so the site reads the same on both strands and the enzyme can cut both strands at the same place

Animated diagram of Name to DNA, from typed letters to a FASTA file
How the data flows, from your name to the DNA, the peptide maths and the FASTA file
Why

To teach people about biology